Total erk antibody

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August undefined, 2024

WebMay 1, 2009 · For total ERK, membranes were stripped for 30 min in a solution of 62.5 mM Tris HCl, 2% SDS, and 100 mM β-mercaptoethanol, and reprobed using a mouse monoclonal antibody to total ERK (1:1000, #4696, Cell Signaling Technology, Beverly, MA) followed by an Alexa 680 conjugated goat anti-mouse secondary antibody (1 : 10 000, Molecular Probes). WebApr 5, 2024 · No significant differences of total ERK protein level were observed in U0126 conditions compared to non ... Membranes were stripped and reprobed with anti-pan-ERK antibody or with anti-α-tubulin ...

Anti-ERK1 + ERK2 antibody (ab196883) Abcam

WebOct 22, 2024 · (C) Levels of p-Erk, Erk and bax in the control group, vehicle group, anti-TNF-α antibody group and anti-TNF-α antibody+PMA group were detected by Western blotting. ( … WebThe Phospho/Total ERK1/2 Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-ERK1/2 (Thr202/Tyr204; Thr185/Tyr187) and total ERK1/2 in human, mouse, and rat whole cell lysates. For Research Use Only. ohio river flow chart

ERK1/2 Monoclonal Antibody (5AD13MA) (14-9108-82)

WebApr 12, 2024 · HIGD2A promoted liver cancer cell growth by fueling mitochondrial ATP synthesis and activating the MAPK/ERK pathway, ... USA) for immunoblotting with the following primary antibodies: anti-HIGD2A antibody (ab150893, Abcam, USA), anti-Mfn1 antibody (13798-1-AP, ProteinTech ... Total RNA was extracted using the EZ-press RNA ... WebThe assay will exclusively recognize both natural and recombinant ERK 1/2, when phosphorylated at Threonine 202 and Tyrosine 204. Principle of the method The phospho ERK1/2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. WebNov 13, 2024 · Phosphorylated ERK 1 and ERK2 were detected with anti-DP-ERK antibody (α-DP-ERK). The amounts of total ERK1 and ERK2 were detected with anti-ERK antibody (α-G-ERK). The results in the bar graph represent the percent activation of that obtained in the GnRH-a-stimulated cells that were co-transfected with GnRHR and vector control in each ... ohio river hotels in indiana

Anti-ERK2 Antibodies Invitrogen - Thermo Fisher Scientific

HIGD2A silencing impairs hepatocellular carcinoma growth via …

Webone 96-well assay with the phospho-ERK antibody and one 96-well assay with the total-ERK antibody. 9.1 1X PBS: Prepare 1X PBS by adding 1 volume of 10X PBS (pH 7.4) to 9 volumes of dH2O and mixing thoroughly. 9.2 Preparation of Fixing Buffer (4% or 8% Formaldehyde in PBS): Fixing Buffer is used to fix cells after cell culturing. Prepare by WebHRP conjugated antibodies were used as the secondary antibodies. Data is expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) … myhome fortimoWebAug 31, 2024 · The proteins were extracted and subjected to SDS-PAGE and Western blotting analysis. Anti-phosphorylated ERK and total ERK antibodies were used to analyze ERK protein. The fold changes were indicated under the phosphorylated and total ERK bands relative to the GFP siRNA baselines. C-H, DENV2 titers is increased in the Ras knockdown … ohio river freeze

"WebSep 1, 2011 · The antibody against ERRγ was raised in rabbits against an NH 2-terminal peptide corresponding to amino acids 24–40 of the mouse ERRγ protein conjugated to keyhole limpet hemocyanin ... Bottom left: quantitation of p-ERK vs. total ERK was performed, and graphs depict means ± SE of 3 independent experiments. " - Total erk antibody

Total erk antibody

ERK 1 Antibody (K-23) SCBT - Santa Cruz Biotechnology

WebSee: MAPK/ERK pathway. Transgenic gene knockout mice lacking MAPK1 have major defects in early development. Conditional deletion of Mapk1 in B cells showed a role for MAPK1 in T-cell-dependent antibody production. A dominant gain-of-function mutant of Mapk1 in transgenic mice showed a role for MAPK1 in T-cell development. WebOct 22, 2024 · (C) Levels of p-Erk, Erk and bax in the control group, vehicle group, anti-TNF-α antibody group and anti-TNF-α antibody+PMA group were detected by Western blotting. ( D ) Quantitative analyses showing the levels of p-Erk relative to total Erk, and bax relative to β-actin normalized to sham-operated control group (n=5/group, ** p <0.01 versus sham …

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WebCell Signaling Technology Inc anti total erk antibody Anti Total Erk Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebThe cells were labeled with ERK1 + ERK2 Antibody [ERK-7D8] (ab54230) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 …

Web19 rows · Erk Antibody Comparison Table. Phospho-Specific Protein p44/42 MAPK (Erk 1/2) Antibody Comparison Table. No. Antibody Reactivity WB IP IHC Flow IF; No. Antibody: … WebJul 27, 2000 · a, Following stimulation with crosslinked anti-CD3 (KT-3) and anti-CD4 (GK1.5) antibodies, we analysed induction of ERK, JNK and p38 phosphorylation by sequential …

WebERK 1 Primary Antibody for Size Assays Erk 1 Rabbit Primary Antibody, 1X stock (1 mL) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. 042-488 HeLa Lysate Controls HeLa lysate, lysed in RIPA Lysis Buffer (20 µL/vial) for use on Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems. 043-311 Luminol-S WebMonoclonal Antibody for studying ERK1/ERK2. Cited in 5673 publications. Validated for WB, IP, IHC, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, p44/42 MAPK (Erk1/2) (137F5) Rabbit …

WebIn another experiment, cells were pretreated with 2 mg/mL integrin β1-neutralising antibody, total protein was extracted using lysis buffer to analyze FAK, p-FAK, Erk, pErk, Elk1, ... Erk, and Elk1 relative to total FAK, Erk, and Elk1 were gradually increased from 0.5 h to 12 h after IGFBP2 treatment, and then decreased from 12 h to 48 h ...

Webp-ERK 1/2 (Thr202/Tyr204) + Total ERK . assay. The Alpha SureFire Ultra Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous extracellular signal“ regulated - kinase 1 and 2” (ERK 1/2)in cellular lysates. The assay is an ideal system for the screening of ohio river freezingWebFeb 5, 2024 · ERK 1/2 Antibody (C-9) is a mouse monoclonal IgG 2a κ ERK 1/2 antibody, cited in 460 publications, provided at 200 µg/ml. raised against amino acids 101-172 … ohio river frozenWeb9/28/2024 Good Total ERK Antibody YONG M This antibody was used to detect total ERK expression in prostate cell line PNT2, PC3 and ... Read Review (1) Write a Review. Santa … ohio river in kentuckyWebSpecificity / Sensitivity. p44/42 MAP Kinase (L34F12) Mouse mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some systems this antibody may … ohio river forecast shawneetown ilWebThis antibody recognizes Human, Mouse, Rat, Bovine, C. elegans/Worm, Drosophila/Arthropod, Hamster, Non-Human Primate, Porcine, Zebrafish/Fish, and Other Mammalian antigen. The Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody has been validated for the following applications: Precipitation, Flow Cytometry, … ohio river houseboat rentalsWebActivation of Erk (extracellular signal regulated kinase) requires the phosphorylation of Thr202 and Tyr204 amino acid residues. NIH/3T3 cell pellets (Figure A) treated with either … ohio riverfront property for sale in kentucky ohio river gauge paducah