Phosphopantetheine binding
WebUsing Escherichia coli LipB as a model enzyme, we show here that the octanoyl-transferase mainly recognizes the 4'-phosphopantetheine-tethered acyl-chain of its donor substrate and weakly binds the apo-acyl carrier protein. We demonstrate LipB can accept octanoate from its own ACP and noncognate ACPs, as well as C8-CoA. Phosphopantetheine, also known as 4'-phosphopantetheine, is a prosthetic group of several acyl carrier proteins including the acyl carrier proteins (ACP) of fatty acid synthases, ACPs of polyketide synthases, the peptidyl carrier proteins (PCP), as well as aryl carrier proteins (ArCP) of nonribosomal peptide synthetases (NRPS). It is also present in formyltetrahydrofolate dehydrogenase.
Phosphopantetheine binding
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Webblocking the malonyl and the CoA/ACP phosphopantetheine-binding sites (10, 13). A wealth of knowledge about the structure, function, and inhibition of the distributed FAS type II system has been gathered over the last decade (14, 15). However, detailed structural studies of the large eukaryotic FAS type I multifunc- WebMercaptoacetamide: A promising zinc-binding group for the discovery of selective histone deacetylase 6 inhibitors By Maurício T. Tavares
Web4'-Phosphopantetheine C11H23N2O7PS CID 115254 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, … WebCrystal structures of the PCP-reductase didomain reveal that phosphopantetheine binding alters the orientation of a key Asp, resulting in a productive orientation of the bound nicotinamide. This ensures that further reduction of the aldehyde product does not occur.
WebApr 16, 2014 · Protein has 1 cofactor binding site: pantetheine 4'-phosphate ( UniProtKB Rhea CHEBI:47942 ) By similarity Note: Binds 2 phosphopantetheines covalently. By similarity Pathway Siderophore biosynthesis; mycobactin biosynthesis. GO annotations Expand table Complete GO annotation on QuickGO Keywords Molecular function # Ligase WebFeb 1, 2024 · The reaction mechanism of PPAT involves a nucleophilic attack by 4′-phosphate of phosphopantetheine on the α-phosphate of ATP in an in-line displacement …
WebNov 9, 1999 · The first domain (AL) shows 49% similarity to long-chain fatty acid CoA-ligases and contains a putative ATP binding box. The second and fourth domains (ACP) show 45% similarity to acyl carrier proteins, and both contain a putative 4′-phosphopantetheine binding box. The third domain (KS) is 53% similar to β-ketoacyl synthetases. high back beach chair with cup holderWeb2.8 2.9 Structures of essential biomolecules pantothenate, phosphopantetheine, coenzyme A, and acyl-ACP Biosynthetic pathway of Coenzyme A Sequence similarity of CoA biosynthetic enzymes among several species Selected CoA biosynthetic pathway inhibitors The membranes of gram-negative bacteria Biosynthetic pathway of lipid A how far is it from marblehead ma to salem maWebJan 4, 2024 · Crystal structures of the PCP-reductase didomain reveal that phosphopantetheine binding alters the orientation of a key Asp, resulting in a productive orientation of the bound nicotinamide. This ensures that further reduction of the aldehyde product does not occur. Combining crystallography with small-angle X-ray scattering … how far is it from marbella to sevilleWebApr 15, 1999 · The hydrogen bond between the amide of Met74 and the carboxylic oxygen at the elbow of the pantetheine arm stabilizes the bent configuration. In the free protomer, residues 72–74 hinder binding of 4′-phosphopantetheine and would result in steric conflict between Met74 and carboxylic oxygen, thus entailing backbone movement prior to binding. high back beach chairs for seniorsWebOct 5, 2007 · Together with docking simulations that identified a potential phosphopantetheine binding groove, the structural and functional studies reveal that the C9 specificity is a result of active site geometry and substrate ring constraints. The results lay the foundation for the design of novel aromatic polyketide natural products with different ... high back bathing suits for womenWebFeb 9, 2024 · Binding poses of the natural substrates 4′-phosphopantetheine (PPSH) and ATP are shown for comparison. C) Hydrogen-bonding interactions and hydrophobic … high back beach chair saleWebPhosphopantetheine-binding protein (Streptomyces rimosus subsp. rimosus ATCC 10970) Cite Download Contents 1 Sequence 2 Interactions and Pathways 3 Biochemical … high back bathing suit