Brood size assay
WebBrood size assay The number of live progeny produced daily can also be used to study reproduction. Wild-type hermaphrodites use their limited number of self-sperm and can … WebJul 15, 2024 · Brood size assay. The brood size assay was conducted as previous described (Craig et al., 2012; Shinya et al., 2014). The him-5 male mutants were exposed to the SMF, then him-5 male mutants were picked into a new NGM plate containing OP50, one for each plate, and then a female fog-2 mutant was selected into the new plate.
Brood size assay
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WebBrood size assay. The number of live progeny produced daily can also be used to study reproduction. Wild-type hermaphrodites use their limited number of self-sperm and can … WebBrood size assays quantify the number of eggs laid over the worm's reproductive lifetime 4. If the brood size of worms in the experimental and control groups are the …
WebThis experiment determined whether the assay is capable of detecting genetic differences in brood care behavior by comparing the performance of bees from different genetic … WebA change from 1.0 × 10 12 cfu/mL to 1.0 × 10 8 cfu/mL resulted in a reduction in brood size from ∼200 to ∼50, but with no observable change in the length of the reproductive period. Houthoofd et al. (2002) observed that culture in axenic liquid media reduced brood size and extended the reproductive period.
WebJan 21, 2014 · In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and … WebSep 9, 2024 · Brood size assay. L4-stage mothers were trained for 24 h on control (empty vector) or P11-expressing E. coli as described in ‘Worm preparation for training’. After 24 h, 15 individual worms ...
WebBrood: Bee Babies. Brood is a catch-all term for baby bees in various stages of their development cycle, from egg to fully-functioning adult. When the queen lays an egg, it …
WebThe brood size assay can be performed by either determining the complete brood size (total number of progeny) over a period of 5–6 d or by determining a partial brood size after 3 … green day oh love testoWebJul 15, 2024 · In addition, the brood size assay revealed that 10 T SMF exposure eventually diminished the reproductive capacity of him-5 male worms. The lifespan of outcrossed offspring from exposed him-5 male mutants and unexposed fog-2 female mutants was decreased by 10 T SMF in a time dependent manner. Together, our … green day oh love mp3WebBrood size assay of C. elegans using different concentrations of the purified YqfO03. The assay was measured in 3 d. Error bars represent the standard deviations from the mean … fls schulportalWebOct 8, 2013 · The brood size assay was set up in 96-well microtiter plates with each well containing a single L4 worm, 10 μL of mixture YEC-10 and YEC-11 in S medium, and 110 μL of S medium bring total volume to 120 μL (Wei et al. 2003). Five wells were assayed for each concentration. Microtiter plates were inoculated in a plastic box containing a damp ... green day official websiteWebthe lethal dose assay. The brood size assay can be performed by either determin-ing the complete brood size (total number of progeny) over a period of 5–6 d (22) or by … fls scaffoldingWebAug 23, 2024 · (E) brood size assay of homozygous mex-3(spr6) mutant animals at 20°C.There is no reduction in the brood size. (F) brood size assay of homozygous mex-3(spr7) mutant animals at 20°C. Each dot in panels E and F represents the brood size of an individual animal. In each panel, data from three biological replicates are shown in the … flss accessorieshttp://nemaplex.ucdavis.edu/Methods/Agar%20Culture.htm fls scheduling